WebDec 20, 2016 · Using the PureWheat technique, another model spring wheat genotype, Fielder, was successfully transformed (Ishida et al., 2015). Recently, Richardson et al . ( 2014 ) achieved Agrobacterium ‐mediated transformation of eight Australian and Mexican commercial spring wheat varieties, among which Westonia and Gladius showed effective … WebJul 11, 2014 · A previously reported Agrobacterium tumefaciens transformation system that transformed wheat cultivar Fielder at high efficiency was shown to also transform eight …

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WebNov 10, 2016 · The Japan Tobacco Company developed what they call the PureWheat technique for the spring wheat variety Fielder is used (Ishida et al., 2015). Employing this PureWheat technique, Richardson et al. successfully transformed two Australian commercial wheat varieties, Westonia and Gladius, with efficiencies of 45% and 32%, … WebJan 13, 2024 · However, PureWheat still has limitation in transforming many commercialized wheat cultivars which show strong genotype-dependency, being low efficiencies or recalcitrant to transformation even using PureWheat 2,3. Therefore, overcoming genotype-dependence in transformation is still needed for wheat improvement. osu on campus housing

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