WebLysoTracker Green DND-26 is a cell-permeable, non-fixable, green fluorescent dye that stains acidic compartments within a cell, such as lysosomes. Green DND-26 has an excitation and emission maximum of 504/511 nm and can be efficiently excited using a FITC filter. LysoTracker probes are available in a variety of fluorescent colors. Web3 nov. 2024 · Lysosome-565 co-localized well with both LysoTracker Green (the most popular commercially available organic fluorescent acidotropic probe for lysosomes, Pearson’s coefficient: 0.776, Fig. 1a)...
Lyso-Tracker Green (溶酶体绿色荧光探针) - Beyotime
Web7 dec. 2024 · Real-time tracking of lysosomes in MCF-7 cells stained with Lyso-Tracker Green (200 nM); images captured per 5 s with an excitation of 488 nm for a total period of 120 s ( AVI) CLSM HSR imaging of the movement of lysosomes with 1 nM DCIP in MCF-7 cells; images captured per 0.44 s with an excitation of 488 nm for a total period of 65 s ( … Web13 apr. 2024 · After incubation, the medium was removed and the cells were washed twice with PBS. LysoTracker Green (50 nm) was then added to dishes to stain the lysosomes of the cells for 30 min before rinsing three times with PBS. The experimental procedure was similar to that described previously (Forteath et al., 2012). 2.6 Intracellular ROS generation county for orangeburg sc
LysoTracker Green DND-26 - Cell Signaling Technology
WebLyso-Tracker Green是嗜酸性荧光探针,用于活细胞内酸性细胞器的标记和示踪。 这些探针具有几个重要特征,包括高度选择靶向酸性细胞器和在纳摩尔浓度有效标记活细胞。 Lyso-Tracker Green必须在极低浓度(通常约50nM)下才能获得优异的选择性。 这些探针的滞留(retention)机制虽然没有被研究清楚,但很可能与酸性细胞器的质子化和滞留性有 … WebStore at -20°C LysoTracker® Green DND-26 n 500 µl (10 x 50 µl) Description: LysoTracker® Green DND-26 is recommended for live cell imaging only; fixation with aldehydes or alcohols will inhibit staining. Excitation: 504 nm Emission: 511 nm Background: LysoTracker® Green DND-26 is a cell perme-able green dye that stains acidic … WebTable 12.3 Summary of our LysoTracker and LysoSensor probes. * Absorption (Abs) and fluorescence emission (Em) maxima, determined in aqueous buffer or methanol; values … county for orlando fl 32837